Women have two Bartholin's glands, located on each side of the entrance to the vagina. The glands make a fluid that helps lubricate the vulva (external female genitalia) and vagina. Sometimes, a What are bartholin glands and their functions in women's intimate health? Genitals Sex and Facts The Bartholin's glands are located on each side of Sukhayu Hospital Nashik The Bartholin's gland is located at the entrance to the vagina, and it secretes mucus, providing vulval and vaginal lubrication. A Bartholin's cyst occurs when the gland becomes blocked, and this can Labia Minora: What Is It, Location, and More Osmosis